Expression of Folliculogenesis-Related Genes in Vitrified Human Ovarian Tissue after Two Weeks In Vitro Culture
نویسنده
چکیده
1. Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2. Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran 3. Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran 4. Department of Biostatistics, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
منابع مشابه
Effect of Human Ovarian Tissue Vitrification/Warming on the Expression of Genes Related to Folliculogenesis
Background: Ovarian tissue cryopreservation is an alternative strategy to preserve the fertility of women predicted to undergo premature ovarian failure. This study was designed to evaluate the expression of folliculogenesis-related genes, including factor in the germline alpha (FIGLA), growth differentiation factor-9 (GDF-9), follicle-stimulating hormone receptor (FSHR), and KIT LIGAND after v...
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Background The high effectiveness of vitrification, as a cryopreservation protocol, for human oocytes and embryos is shown, whereas data on human ovarian tissue are limited. This study aimed to assessment of follicular growth, ultrastructure, and apoptosis incidence in human ovarian tissue following vitrification/warming and after culture in the presence of LIF. MaterialsAndMethods Ovarian cort...
متن کاملP-24: In Vitro Growth and Apoptosis Incidence, valuation of in Vitrified Human Ovarian Tissue Following Treatment with Growth Differentiating Factor 9B (GDF- 9B) and Leukemia Inhibitory Factor (LIF)
Background The conventional freezing and vitrification are different cryopreservation protocols for fertility preservation in cancer patients. The high effectiveness of vitrification for human oocytes and embryos is shown, whereas data on human ovarian tissue are limited. Follicular growth, ultrastructure, and apoptosis incidence in human ovarian tissue following vitrification/warming and after...
متن کاملI-16: Assessment of The Vitrified Ovarian Tissue in Long Term Culture
In vitro culture of human ovarian tissue the following cryopreservation is proposed for follicular development. There are no techniques that guarantee successful maturation of the follicles within the excised tissue. The viability of cultured human ovarian tissue improved by adding some growth factors to the culture media. The efficiency of vitrification as the cryopreservation method for human...
متن کاملHuman Ovarian Tissue Vitrification/Warming Has Minor Effect on the Expression of Apoptosis-Related Genes
Background: In this study, we evaluated the incidence of apoptosis at the ultrastructural levels and expression of some apoptosis-related genes in vitrified human ovarian tissue just after warming. Methods: Human ovarian tissue biopsies from 23 women after caesarean section were transported to the laboratory within 2 hours, and then they were cut into small pieces. Some pieces were vitrified an...
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